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G6PD5.4 is able to establish an OPPP metabolon in ER subdomains To investigate whether G6PD5.4 (1st OPPP step) with a free C-terminal end may recruit both PGL2 (2nd OPPP step) and PGD2 (3rd OPPP step) to a membrane-bound metabolon, a reporter-less expression cassette was also prepared for PGD2 and inserted in front of the GFP-G6PD5.4 cassette (NXn site)

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And if they end up contributing more than they're allowed to as a result, then they'll be forced to withdraw the excess contribution (and any growth attributable to it) or else face a 6% excise tax per year on the excess contribution until it is eventually withdrawn